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Test Code AFBSS AFB STAT SMEAR

Collection Instructions

BODY FLUIDS (excluding urine):

  1. Collect fluid (pleural, peritoneal, etc.) according to standard collection protocol.
  2. Submit a minimum of 5 mL of fluid in a sterile leak proof container.
  3. Deliver to laboratory IMMEDIATELY.

BRONCHOSCOPY SPECIMENS:

  1. Collect bronchial washing or lavage material according to standard protocol.
  2. Submit a minimum of 2 mL of specimen in a sterile leak proof container.
  3. Deliver to laboratory IMMEDIATELY.

EXUDATES:

  1. Aseptically collect drainage with needle and syringe and transfer to sterile leak proof container.
  2. 0.5-1.0 mL sterile water or sterile saline may be added to keep very small specimens moist.
  3. Deliver to laboratory IMMEDIATELY.

SPUTUM:

  1. Obtain a "Sterile Sputum Collector" device.
  2. Instruct patient to expectorate 5-10 mL of material from a first morning, deep cough into container.
  3. Deliver to laboratory as soon as possible; keep refrigerated until delivered.
  4. 24-hour pooled collections ARE NOT acceptable.
  5. Multiple specimens should be collected at least one day apart.

TISSUE:

  1. Obtain sample of tissue according to applicable standard protocol.
  2. Submit actual tissue (lymph node, bone marrow, granuloma, etc.) in sterile, dry container.
  3. Deliver to laboratory IMMEDIATELY.

Collection Container

Sterile dry leak-proof container

Specimen Required/Volume

Source Dependent

Specimen Type

Body Fluids, Bronchoscopy specimens, Exudates, Sputum, Tissue

Turn-Around Guidelines

FREQUENCY: Daily, upon arrival (day shift only)

TAT: 1 day

STAT: Yes

 

Reference Values

NO ACID FAST BACILLI SEEN

Method

Fluorescent Microscopy

CPT

87206 - AFB Smear

87015 - Specimen concentration

 

LOINC

11545-1