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Test Code MHRP Metamycoplasma hominis, Molecular Detection, PCR, Varies

Reporting Name

Mycoplasma hominis PCR

Useful For

Rapid, sensitive, and specific identification of Metamycoplasma hominis from synovial fluid, genitourinary, reproductive, lower respiratory sources, pleural/chest fluid, pericardial fluid, and wound specimens

 

This test is not intended for medicolegal use.

Method Name

Real-Time Polymerase Chain Reaction (PCR) using LightCycler and Fluorescent Resonance Energy Transfer (FRET)

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Varies


Necessary Information


Specimen source is required.



Specimen Required


The high sensitivity of amplification by polymerase chain reaction (PCR) requires the specimen to be processed in an environment in which contamination of the specimen by Mycoplasma hominis DNA is not likely.

 

Submit only 1 of the following specimens:

 

Specimen Type: Swab

Supplies:

-Culturette (BBL Culture Swab) (T092)

-BD E-Swab (T853)

-M4-RT (T605)

Sources: Vaginal, cervix, urethra, urogenital, chest/mediastinal; bronchus or lung (donor swab); or upper respiratory sources (only infants <3 months: nasopharynx, nose, throat)

Container/Tube:

Preferred: Culture swab transport system (Dacron or rayon swab with aluminum or plastic shaft with either Stuart or Amies liquid medium)

Acceptable: Swab in transport media: M4, M4-RT, M5, M6, universal transport media, or ESwab

Specimen Volume: 1 swab

Collection Instructions:

Vaginal:

1. Collect specimen by swabbing back and forth over mucosa surface to maximize recovery of cells.

2. Place swab back into swab cylinder.

Urethra or cervical:

1. Collect specimen by inserting swab 1 to 3 cm and rotating 360 degrees.

2. Place swab back into swab cylinder.

Wound:

1. Collect specimen by swabbing back and forth over wound surface to maximize recovery of cells.

2. Place swab back into swab cylinder.

 

Specimen Type: Fluid

Sources: Pelvic, peritoneal, amniotic, prostatic secretions, semen, reproductive drainage or fluid, pleural/chest, chest tube, pericardial

Container/Tube: Sterile container

Specimen Volume: 1 to 2 mL

 

Specimen Type: Respiratory

Sources: sputum, tracheal secretions, bronchial washings, bronchoalveolar lavage, lung; or nasal washings (Note: Nasal washings may only be submitted for infants <3 months of age.)

Container/Tube: Sterile container

Specimen Volume: 1 to 2 mL

 

Specimen Type: Synovial fluid

Container/Tube:

Preferred: Lavender top (EDTA)

Acceptable: Pink top (EDTA), royal blue top (EDTA), sterile vial containing EDTA-derived aliquot, red clot tube (no anticoagulant), or sterile container

Specimen Volume: 0.5 mL

Collection Instructions: Send specimen in original tube.

 

Specimen Type: Urine (first void), kidney/bladder stone, or ureter

Container/Tube: Sterile container

Specimen Volume: 10 mL or entire specimen

Collection instructions: Urine first void: Specimen can be collected at any time during the day. The patient should not have urinated for at least 1 hour prior to specimen collection. The first voided portion is the initial 20 to 30 mL of the urine stream obtained without cleaning the external urethra.

 

Specimen Type: Tissue

Sources: Placenta, products of conception, urogenital, respiratory, bronchus, chest/mediastinal, bone, spine, or joint

Container/Tube: Sterile container

Specimen Volume: 5 mm(3)

Collection Instructions:

1. Collect fresh tissue specimen.

2. Submit fresh tissue only, do not add fluid to tissue

3. Refrigerate or freeze specimen.


Specimen Minimum Volume

Fluid: 1 mL
Urine, first void: 2 mL
Swab: 1 swab
Tissue: 5 mm(3)

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Refrigerated (preferred) 7 days
  Frozen  7 days

Reject Due To

Cotton or calcium alginate-tipped swab
Wooden shaft swab
Transport swab containing gel or charcoal
Formalin-fixed and/or paraffin-embedded tissues,
Port-a-Cul tube
Anaerobic fluid vials
Dry swab (no pledget or sponge)
Bone marrow
Decalcified bone
Slides
Respiratory fluid specimens placed in viral transport medium (VTM) or placed on a swab and then in VTM (M4-RT, M4, or M5)
Body fluid specimens placed in viral transport medium (VTM) or placed on a swab and then in VTM (M4-RT, M4, or M5)		 Reject

Reference Values

Not applicable

Day(s) Performed

Monday through Friday

CPT Code Information

87798

LOINC Code Information

Test ID Test Order Name Order LOINC Value
MHRP Mycoplasma hominis PCR 68546-1

 

Result ID Test Result Name Result LOINC Value
SRC86 Specimen source 31208-2
32536 Mycoplasma hominis PCR 68546-1

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

Report Available

3 to 4 days

NY State Approved

Yes

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.