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Test Code MP8BS Mucopolysaccharidoses, Eight-Enzyme Panel, Blood Spot


Ordering Guidance


To evaluate newborn patients in follow-up to an abnormal newborn screen for MPSI, the recommended tests are IDUAW / Alpha-L-Iduronidase, Leukocytes and MPSBS / Mucopolysaccharidosis, Blood Spot, MPSWB / Mucopolysaccharidosis, Blood, MPSER / Mucopolysaccharides Quantitative, Serum or MPSQU / Mucopolysaccharides Quantitative, Random, Urine.

 

To evaluate newborn patients in follow-up to an abnormal newborn screen for MPSII, the recommended tests are I2SB / Iduronate-2-Sulfatase, Blood Spot or I2SWB / Iduronate-2-Sulfatase, Leukocytes and MPSBS / Mucopolysaccharidosis, Blood Spot, MPSWB / Mucopolysaccharidosis, Blood, MPSER / Mucopolysaccharides Quantitative, Serum or MPSQU / Mucopolysaccharides Quantitative, Random, Urine.



Necessary Information


1. Patient's age is required.

2. Reason for testing is required



Specimen Required


Submit only 1 of the following specimen types:

 

Preferred:

Specimen Type: Blood spot

Supplies: Card-Blood Spot Collection (Filter Paper) (T493)

Container/Tube:

Preferred: Blood Spot Collection Card

Acceptable: Whatman Protein Saver 903 Paper, PerkinElmer 226 filter paper, Munktell filter paper, or blood collected in tubes containing ACD or EDTA and dried on filter paper.

Specimen Volume: 2 Blood spots

Collection Instructions:

1. An alternative blood collection option for a patient 1 year of age or older is a fingerstick. For detailed instructions, see How to Collect Dried Blood Spot Samples.

2. At least 2 spots should be complete (ie, unpunched).

3. Let blood dry on filter paper at room temperature in a horizontal position for a minimum of 3 hours.

4. Do not expose specimen to heat or direct sunlight.

5. Do not stack wet specimens.

6. Keep specimen dry.

Specimen Stability Information: Refrigerated (preferred) 60 days/Ambient 7 days/Frozen 60 days

Additional Information:

1. For collection instructions, see Blood Spot Collection Instructions

2. For collection instructions in Spanish, see Blood Spot Collection Card-Spanish Instructions (T777)

3. For collection instructions in Chinese, see Blood Spot Collection Card-Chinese Instructions (T800)

 

Acceptable:

Specimen Type: Whole Blood

Container/Tube:

Preferred: Lavender top (EDTA)

Acceptable: Yellow top (ACD)

Specimen Volume: 2 mL

Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.

Specimen Stability Information: Refrigerate (preferred) 7 days/Ambient 48 hours


Forms

1. New York Clients-Informed consent is required. Document on the request form or electronic order that a copy is on file. The following documents are available:

-Informed Consent for Genetic Testing (T576)

-Informed Consent for Genetic Testing-Spanish (T826)

2. Biochemical Genetics Patient Information (T602)

3. If not ordering electronically, complete, print, and send a Biochemical Genetics Test Request (T798) with the specimen.

Useful For

Supporting the biochemical diagnosis of mucopolysaccharidoses types II, IIIA, IIIB, IIIC, IVA, IVB, VI, and VII, and of multiple sulfatase deficiency

 

This test is not useful for carrier detection.

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
MPSBS Mucopolysaccharidosis, BS Yes No

Testing Algorithm

Testing begins with screening for mucopolysaccharidoses. If results are normal or indicate mucopolysaccharidosis VII, testing is complete.

 

If results indicate mucopolysaccharidoses II, IIIA, IIIB, IIIC, IVA, IVB, or VI, quantitation of heparan sulfate, dermatan sulfate and keratan sulfate may be performed at an additional charge.

Method Name

Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

Reporting Name

MPS (Eight) Panel, BS

Specimen Type

Whole blood

Specimen Minimum Volume

Blood Spots: 1
Whole Blood: 0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole blood Varies

Reject Due To

Blood spot specimen that shows serum rings or has multiple layers Reject
Insufficient specimen Reject
Unapproved filter papers Reject

Reference Values

Iduronate-2-sulfatase: >4.30 nmol/mL/hour

Heparan-N-sulfatase: >0.06 nmol/mL/hour

N-acetyl-alpha-D-glucosaminidase: >0.70 nmol/mL/hour

Heparan-alpha-glucosaminide N-acetyltransferase: >0.50 nmol/mL/hour

N-acetylgalactosamine-6-sulfatase: >0.70 nmol/mL/hour

Beta-galactosidase: >1.30 nmol/mL/hour

Arylsulfatase B: >0.90 nmol/mL/hour

Beta-glucuronidase: >2.60 nmol/mL/hour

 

An interpretive report will be provided.

Day(s) Performed

Thursday

Report Available

8 to 15 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

82657

83864 (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
MP8BS MPS (Eight) Panel, BS 104112-8

 

Result ID Test Result Name Result LOINC Value
BG743 Reason for Referral 42349-1
618405 Iduronate-2-sulfatase 79462-8
618406 Heparan-N-sulfatase 104113-6
618407 N-acetyl-alpha-D-glucosaminidase 104114-4
618408 Heparan-alpha-glucosaminide N-acetyltransferase 104115-1
618409 N-acetylgalactosamine-6-sulfatase 88019-5
618410 Beta-galactosidase 55916-1
618411 Arylsulfatase B 55912-0
618412 Beta-glucuronidase 79457-8
618413 Interpretation 59462-2
618404 Reviewed By 18771-6

NY State Approved

Yes