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Test Code PLAFL Platelet Surface Glycoprotein by Flow Cytometry, Blood

Useful For

Identification of markedly decreased CD41 (GPIIb) and CD61 (GPIIIa) expression levels, which are diagnostic for Glanzmann thrombasthenia

 

Identification of markedly decreased CD42a (GPIX) and CD42b (GPIb-alpha) expression levels, which are diagnostic for Bernard-Soulier syndrome

 

Identification of decreased GPVI expression, which suggests collagen receptor deficiency

 

Identification of decreased CD49b (GPIa), which suggests collagen receptor deficiency

Method Name

Immunophenotyping

Reporting Name

Platelet Glycoprotein Flow, B

Specimen Type

Whole Blood ACD


Shipping Instructions


Specimen must be shipped ambient and arrive within 4 days of draw.

 

Ship specimen overnight in an Ambient Shipping Box-Critical Specimens Only (T668) following the instructions in the mailer.



Necessary Information


Platelet Esoteric Testing Patient Information is required. Testing may proceed without the patient information, however, the information aids in providing a more thorough interpretation. Ordering providers are strongly encouraged to fill out the form and send with the specimen.



Specimen Required


Supplies: Ambient Shipping Box-Critical Specimens Only (T668)

Collection Container/Tube: ACD solution A or B

Specimen Volume: 6 mL

Pediatric Volume: 1 mL

Collection Instructions: Do not transfer blood to other containers.


Specimen Minimum Volume

Adult: 1 mL
Pediatric 200 mcL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole Blood ACD Ambient 4 days

Reject Due To

Gross hemolysis Reject
Fully Clotted Reject
Gross lipemia OK

Reference Values

GPIIb CD41: ≥70.0% (Normal Range-Median)

GPIIIa CD61: ≥70.0% (Normal Range-Median)

GPIX CD42a: ≥70.0% (Normal Range-Median)

GPIb-alpha CD42b: ≥70.0% (Normal Range-Median)

GPIa CD49b: ≥60.0% (Normal Range-Median)

Day(s) Performed

Monday through Saturday

Report Available

1 to 2 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

88184-Flow cytometry; first cell surface, cytoplasmic or nuclear marker

88185-Flow cytometry; additional cell surface, cytoplasmic or nuclear marker (each) X5

88187-Flow cytometry interpretation, 2 to 8 markers

LOINC Code Information

Test ID Test Order Name Order LOINC Value
PLAFL Platelet Glycoprotein Flow, B 93320-0

 

Result ID Test Result Name Result LOINC Value
CK111 GPIIb CD41 93319-2
CK112 GPIIIa CD61 93318-4
CK113 GPIX CD42a 93317-6
CK114 GPIb-alpha CD42b 93316-8
CK115 GPIa CD49b 93315-0
CK116 GPVI 93314-3
CK117 Final Diagnosis 93313-5

NY State Approved

Yes

Forms

1. Platelet Esoteric Testing Patient Information is required.

2. If not ordering electronically, complete, print, and send a Coagulation Test Request (T753) with the specimen.